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3.Detection of In-Vitro Antibacterial Activity of Tinospora Cordifolia and Solanum Nigrum Leaf Extracts against Multidrug Resistant- Escherichia Coli
Sehrish Mahmood1, Muhammad Owais Ismail1, Akhtar Ali1, Jabbar Ahmed1, Faisal Iqbal Afridi2 and Humaira Arif3
ABSTRACT
Objective: It was to determine the in-vitro antibacterial activity of ethanolic leaf extracts of Tinospora cordifolia (TC) and Solanum nigrum (SN) against Multidrug resistant- Escherichia coli (MDR-E.coli).
Study Design: In-vitro experimental study.
Place and Duration of Study: This study was conducted at the Pharmacology Department, Ziauddin University, Karachi from December 2022 to May 2023.
Materials and Methods: TC and SN leaves were extracted using a rotary evaporator. Antibacterial sensitivity of both extracts was determined by Agar well diffusion assay and the Broth Dilution assay.
Results: Total 8 concentrations from 3.90 to 500 mg/ml were made in 10% DMSO for both the extracts. No significant antibacterial activity was observed against MDR-E.coli at any tested concentration.
Conclusion: The leaf extracts of Solanum nigrum and Tinospora cordifolia were ineffective against MDR- E.coli of DFI.
Key Words: DFI, antibacterial activity, MIC, MDR- E.coli, Solanum nigrum leaf extract, Tinospora cordifolia leaf extract