9. Vitamin Thiamine and Glucose Homeostasis in Alloxan Induced Diabetes Mellitus
Abdul Hafeez Baloch1, Iqbal Ahmed Memon2 and Kashif Rasheed Shaikh3
Objective: valuating the vitamin B1 (thiamine) on glucose homeostasis and Glycosylated hemoglobin A1 (HbA1c) in Alloxan induced diabetic rat model.
Study Design: Experimental study
Place and Duration of Study: This study was conducted at the Animal House, Isra University Hyderabad from September 2014 to March 2015.
Materials and Methods: 60 adult albino rats were divided into four groups; Group 1. Controls, Group 2. Rats receiving thiamine added diet, Group 3. Diabetics rats on normal diet and Group 4. Diabetic rats receiving thiamine added diet. Alloxan (120 mg/kg) was introduced intraperitoneally to induce diabetes. Vitamin B1 was given orally at 1.6 g/kg body weight for 12 weeks. Venous blood was taken from tail vein by small bore cannula at the baseline and after 12th week. Blood glucose and HbA1c were detected at baseline and after 12th week. Data was saved in proforma and analyzed on SPSS 22.0 using paired student t-test at 95% confidence interval.
Results: Blood glucose and HbA1c levels were found statistically significant in grou s 1 vs. 3 (p=0.0001), 1 vs. 4 (p=0.0001), 2 vs. 3 (p=0.0001), 2 vs. 4, (p=0.001) and 3 vs. 4 (p=0.024) at the end of experiment period. Significant improvement in blood glucose and HbA1c was noted in the vitamin thiamine treated rats.
Conclusion: Vitamin thiamine improved the blood glucose homeostasis and reduced Glycosylated Hemoglobin A1 effectively in experimental rats. It is recommended to supplement diabetic subjects with vitamin thiamine. Key Words: Vitamin thiamine, Glucose homeostasis, HbA1c
Citation of article: Baloch AH, Memon IA, Shaikh KR. Vitamin Thiamine and Glucose Homeostasis in Alloxan Induced Diabetes Mellitus. Med Forum 2016;27(6):36- 38.