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  3. 5. Association of PD-1 and PDL-1 Gene Polymorphisms in Type 2 Diabetes with Toxoplasmosis
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5. Association of PD-1 and PDL-1 Gene Polymorphisms in Type 2 Diabetes with Toxoplasmosis

Original Article

PD-1 and PDL-1 Gene Polymorphisms with Toxoplasmosis in Type 2 Diabetes

Association of PD-1 and PDL-1 Gene Polymorphisms in Type 2 Diabetes with Toxoplasmosis

Farah E. Mohammed1, Ali N. Yaseen2 and Muhammed A. H. Aldabagh3

ABSTRACT

Objective: To investigate the relationship between PD1 and PDL-1 gen polymorphisms with incidence toxoplasmosis in type 2 diabetes disease (T2DM).

Study Design: Case control study

Place and Duration of Study: This study was conducted at the Al-Iamamain Al-Kadhumain Teaching Hospital, Baghdad Iraq from 1st December 2018 to 30th April 2019.

Methods: One hundred and eighty patients with Type 2 diabetes mellitus (T2DM) aged 31-79 years, attending the diabetes were enrolled. A control group of 163 healthy subjects was also included. The PCR products of the SNPs were sequenced using the Big Dye Terminator method and compared to sequences in GenBank.

Results: Extracted DNA were used for molecular study for determination of the (PD-1.5 C/T rs 2227981) and the (PDL-1 GCA rs1970000)  gene polymorphisms by conventional PCR method using specific primer. Sequencing of PCR products revealed three genotypes for the PD-1.5 C/T(CC, CT, TT). Regarding PD-1 showed no significant difference in each CT and CC genotypes between case and control groups while TT genotype showed significant difference between them. As well as related to PDL-1 showed significant difference between case and control while, CC showed non-significant between case and control groups. A allele significantly higher frequency in controls than cases. In contrast, C allele non-significantly higher frequency in cases than controls

Conclusion: There is a significant association between the phenotypic polymorphism of the (PD-1.5 C/T gene rs2227981) Toxoplasmosis and type 2 diabetes for the groups in the current study, as it was found that people who carry the genotype TT are more likely to develop type 2 diabetes than people who carry the genotype CC.

Key Words: PD-1, PDL-1, Polymorphisms, Toxoplasmosis, Type 2 Diabetes

Citation of article: E-Mohammed F,  Yaseen AN, AH Aldabagh M. Association of PD-1 and PDL-1 Gene Polymorphisms in Type 2 Diabetes with Toxoplasmosis. Med Forum 2024;35(12):23-28.doi:10.60110/ medforum.351205.