4.Role of Transferrin Receptor Protein in Cancer Treatment
Quratulain Maqsood1, Esha Ameen2, Aleena Sumrin1 and Hina Saleem1
ABSTRACT
Objective: This article aims to show the association of the transferrin receptors with cancer and take it as the target in cancer therapy.
Study Design: Prospective Experimental research study
Place and Duration of Study: This study was conducted at the Center for Applied Molecular Biology (CAMB) Punjab University Pakistan, from January 2021 till August 2021 for a period of 08 months.
Materials and Methods: Using the CellTiter 96 AQueous Assay (Promega), we determined the 50% growth inhibition values for the substances. Membrane proteins solubilized and separated on SDS PAGE, dyed, and digested in-gel using conventional methods. HPLC was used to identify the proteins. cDNA was generated using the Retroscript cDNA synthesis kit (Ambion). Time-delayed fluorescence was measured using a Wallac Victor plate reader (PerkinElmer) after each well was incubated with Europium-Streptavidin.
Results: Tumor cell apoptosis is triggered by GA binding to TfR. The results of these studies suggest that GA can be used for targeting the TfR in cancer therapy.
Conclusion: Targeting the TfR has been shown to be effective in the treatment of cancers but has shown some cytotoxic effects as well. The wide use of the TfR in the treatment of cancers has helped target specific receptors against cancer cells. In the future, it is expected that targeting TfR for cancer therapy can be improved to overcome the side effects of this therapy and highly target-specific drugs will be produced.
Key Words: Transferrin, Receptor 1, Cancer, Ferric, antibody, Expression
Citation of article: Maqsood Q, Ameen E, Sumrin A, Saleem H. Role of Transferrin Receptor Protein in Cancer Treatment. Med Forum 2021;32(11):16-19.