4.Comparison of Hematoxylin and Eosin Stain with Reticulin and Van Gieson Stain in Confirming Presence, Amount and Type of Bone Marrow Fibrosis
Maliha Asif, Sabeen Fatima, Naseem Akhtar, Ghazala Tabassum, Uzma Ishaq
ABSTRACT
Objective: Comparison of Haematoxylin and Eosin stain, with Reticulin and Van Gieson stain in confirming presence, amount and type of bone marrow fibrosis.
Study Design: Descriptive / cross sectional study
Place and Duration of Study: This study was conducted at the Histopathology and Haematology Department of Sheikh Zayed Hospital, Lahore from Jan 2013 to Dec 2014.
Materials and Methods: Bone marrow trephine biopsies of eighty consecutive patients diagnosed with malignant disorders affecting bone marrow were taken, sections were made and stained with Haematoxylin and Eosin, Reticulin and Van Gieson trichrome stain. Identification and grading of bone marrow was done using European consensus 2005 (EC 2005). Types and grades of fibrosis were reported by using frequency and percentages. Data was entered and analyzed on SPSS 20.
Results: In a total of 80 patient studied, 47 (58.75%) patients showed bone marrow fibrosis on Haematoxylin and Eosin stain, while on Silver/Reticulin stain plus Van Gieson stain, 64(80%) of patients were positive for bone marrow fibrosis. If we grade bone marrow fibrosis on basis of Hematoxylin and Eosin stain ,33 (41.25%) patients had no fibrosis, 25 (31.25%) patients had mild fibrosis, 16(20%) patients had moderate fibrosis, and 6(7.5%) had severe fibrosis. While If we grade bone marrow fibrosis on basis of Silver/Reticulin plus Van Gieson stain using European consensus 2005, 16 (20%) patients had MF-0 (no fibrosis), 40(50%) patients had grade1 fibrosis, 21(26.25%) patients had grade 2 fibrosis, while grade 3 fibrosis was only seen in 3 (3.75%) patients.
Conclusion: Patients with various malignant disorders affecting bone marrow have various grades of bone marrow fibrosis, which can be identified more accurately by using special histochemical stains. Special histochemical stain like Reticulin stain plus Van Gieson stain identified 17 (21.25%) more patients having bone marrow fibrosis which were un-identified on Haematoxylin and Eosin stain.
Key Words: Hematoxylin and Eosin stain, Silver/Reticulin stain, Van Gieson Trichrome stain
Citation of articles: Asif M, Fatima S, Akhtar N, Tabassum G, Ishaq U. Comparison of Hematoxylin and Eosin Stain with Reticulin and Van Gieson Stain in Confirming Presence, Amount and Type of Bone Marrow Fibrosis. Med Forum 2019;30(2):14-18.