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Khalique-ur-Rehman¹, Khalid Shehzad², Syed Muhammad Masood Ali³, Sonia Khan³, Sarah Sughra⁵ and Raja Faisal⁴

ABSTRACT

Objective: To analyze the preventive role of Virgin Coconut Oil (VCO) and Corn Oil (CO) in the testes of rats upon administration of Phenytoin.

Study Design: Analytic study

Place and Duration of Study: This study was conducted at the Al-Tibri Medical College, Karachi from November 2020 to March 2021.

Materials and Methods: 32 male albino rats were acquired by random sampling technique and divided into 4 interventional groups. Group 1 was given Normal Saline Only, Group 2 was given Phenytoin only, Group 3 was given VCO + Phenytoin, and Group 4 was given VO + Phenytoin. The diet, water intake, and light exposure were well regulated and the subjects were then euthanized on the 4^th, 5^th, and 6^th week of the study to assess the germinal layer thickness on the microscope. The testes were removed from the subjects, and tissue samples were acquired and placed on the microscope accordingly and the germinal layer thickness and morphology was assessed at 400x magnification.

Results: Our results showed a significant decrease in the germinal layer thickness in the phenytoin group 2, owing to the generation of oxidative stresses. In the VCO group, a significant improvement was seen compared with the phenytoin group as the germinal layer thickness was restored in this group. Unfortunately, CO wasn’t able to restore the germinal layer thickness and the microscopic findings of the CO group were similar to the phenytoin group. VCO due to its antioxidant potential because of the presence of polyunsaturated fatty acids helps in restoring the germinal layer thickness in the presence of phenytoin.

Conclusion: VCO is a potent antioxidant and can mitigate the toxicity of phenytoin on testes

Key Words: Virgin Coconut Oil, Germinal Layer, Phenytoin, Coconut Oil

Citation of article: Rehman K, Shehzad K, Ali SMM, Khan S, Sughra S, Faisal R. Preventive Role
of Antioxidants in Phenytoin Induced Toxic