3.Diagnostic Utility of Immunohistochemical Expression of Calretinin in Hirschsprung’s Disease
Mohammad Salman Zafar1, Alfarah Rehmatullah3, Nazia Qamar2, Nausheen Ferozuddin2, Nadeem Nusrat2 and Arjumand Imran Baig2
ABSTRACT
Objective: To identify the ganglionic cells in colonic and rectal biopsies by Calretinin immunostain.
Study Design: Experimental and retrospective study
Place and Duration of Study: This study was conducted at the Life care Molecular & PCR Lab Services, Karachi from January 2019 to December 2021.
Materials and Methods: 100 large intestinal biopsy specimens of 50 patients were collected at Life Care Molecular Lab (between 2019-2021) for confirmation of Hirschsprung’s disease and were evaluated with both routine H&E stain and immunohistochemistry using Calretinin.
Results: Children having age from 5 days to 8 years and Male: Female ratio 3:1. 32 cases out of 50 have Hirschprung disease and 16 cases without Hirschprung disease. Haematoxylin and Eosin stain showed false positive results in 25 out of 100 biopsies and 15 cases out of 50. Calretinin showed 100% accuracy in all 100 biopsies studied. Both specificity and accuracy of Calretinin was 92% while H&E showed only 83% and 92% respectively.
Conclusion: Diagnosis of Hirschsprung’s disease on H&E alone may give false positive result resulting in wrong diagnosis and mismanagement. H&E combined with calretinin within aganglionic segment help to eliminate false positive findings during histological examination of specimens for this disease.
Key Words: Ganglionic Cells colonic, Rectal Biopsies, Calretinin immunostain.
Citation of article: Zafar MS, Rehmatullah A, Qamar N, Ferozuddin N, Nusrat N, Baig AI. Diagnostic Utility of Immunohistochemical Expression of Calretinin in Hirschsprung’s Disease. Med Forum 2022;33(5):11-14.