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24.Detection of Helicobacter Pylori from Stomach Biopsies: A Rapid Culture Method

Abdulazeem Abdulsalam Ibrahim Alkhidir1,2, Esraa Hassan Mohammed Ibrahium1, Fatima Suliman Ali Ahmed1, Lubaba Bashieer Alameen Bashieer1, Mashaer Hassan Mohammed Hessen1, Manal Bashieer Alameen Bashieer1, Sarah Hassan Taha Ismaeil1, Alrayah Elsayed Senhuri2, Mohammed Elfatih Hussein Ournasseir1,2, Khalid Mohamed Khalid Elhussain1-3, Ehab Ibrahim Salih El-Amin4 and Khalid Omer Abdalla Abosalif5

ABSTRACT

Objective: To identify a rapid culture method capable of isolating and detecting H. pylori from stomach biopsies.

Study Design: Descriptive / cross-sectional study

Place and Duration of Study: This study was conducted at the Omdurman Islamic University, Sudan from 1st November 2021 to 30th August 2022.

Materials and Methods: The stomach biopsy specimens from 40 patients were collected and centrifugation was done in phosphate buffer saline at 3000 rpm for two minutes. The culture was performed on Modified Brain Heart Urea Agar (MBHUA) media by placing two to three drops of sediment. The cultures were incubated at 37°C in microaerophilic conditions using a candle jar for up to 5 days and observed for growth and change in the color of the medium daily.

Results: Modified Brain Heart Urea Agar media allowed for rapid detection of urease activity in H. pylori within 24 hours after its addition. The bacterial growth was observed three days following the incubation of cultured plates under microaerophilic conditions provided by a candle jar. These results develop rapid detection of H. pylori with clear growth.

Conclusion: H. pylori can be detected more rapidly in modified brain Heart urea agar than the other H. pylori growth media.

Key Words: Helicobacter pylori, Modified Brain Heart Urea Agar, Culture technique

Citation of article: Alkhidir AII, Ibrahium EHM, Ahmed FSA, Bashieer LBA, Hessen MHM, Bashieer MBA, Ismaeil SHT, Senhuri AE, Ournasseir MEH, Elhussain KMK, El-Amin EIS, Abosalif KOA. Detection of Helicobacter Pylori from Stomach Biopsies: A Rapid Culture Method. Med Forum 2022;33(10):105-110.