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  3. 23. Relationship of Oxyresveratrol on Expression Levels of Tumor Necrosis Factor Alpha and Interferon Gamma in Isoniazid Induced Hepatotoxicity in Experimental Model in Mice
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23. Relationship of Oxyresveratrol on Expression Levels of Tumor Necrosis Factor Alpha and Interferon Gamma in Isoniazid Induced Hepatotoxicity in Experimental Model in Mice

Mohammad Abid1, Muhammad Yaqoob Shahani2, Syed Azhar Hussain Zaidi3, Mohammad Anwar Bangulzai6, Shereen Khan4 and Asma Hameed5

ABSTRACT

Objective: To determine the protective effect against isoniazid induced hepatic-toxicity on expression levels of TNF-α and IFN-γ in immunomodulatory activity of oxyresveratrol.

Study Design: Experimental study

Place and Duration of Study: This study was conducted at the Department of Pharmacology, University of Health Sciences, Lahore, Pakistan from 1st July 2019 to 31st December 2019.

Materials and Methods: Mice were given a number from 1 to 35 and were assigned to five groups using lottery method. Each mouse was allotted a number that was written on a piece of small paper. A blinded person picked papers and a mouse whose number was written on paper was assigned group.

Results: The mRNA expression levels of TNF-α were significantly high in INH group as compared to control group (795.9 ± 47.45 vs 417.4 ± 6.55). Oxyresveratrol treatment significantly downregulated expression levels of TNF-α as compared to INH group (372.3 ± 10.27 vs 795 ± 47.45). Oxyresveratrol treatment significantly reduced expression levels of IFN-γ as compared to INH group (225.7 ± 15.57 vs 798 ± 36.9).  Combination therapy showed a significant decreased in expression levels of IFN-γ as compared to INH group (117.7 ± 33.89 vs 798 ± 36.9). Oxyresveratrol showed a significant reduction in interferon γ expression levels as compared to silymarin (225.5 ± 15.57 vs 368.9 ± 22.80).

Conclusion: Oxyresveratrol possesses a protective effect against the isoniazid induced hepatic-toxicity. The decrease in expression levels of TNF-α and IFN-γ may explain immunomodulatory activity of oxyresveratrol.

Key Words: Tumor Necrosis Factor, Alpha, and Interferon Gamma, Isoniazid Induced Hepatotoxicity

Citation of article: Abid M, Shahani MY, Zaidi SZH, Bangulzai MA, Khan S, Hameed A. Relationship of Oxyresveratrol on Expression Levels of Tumor Necrosis Factor Alpha and Interferon Gamma in Isoniazid Induced Hepatotoxicity in Experimental Model in Mice. Med Forum 2021;32(3):100-104.