23. Comparison of Performance Characteristics Between FIA 8000 and Vitros ECiQ Analyzer for Cardiac Troponin I Estimation
Farheen Aslam1, Tariq Arain2, Maria Mehmud3, Zakir Ali4 and Asma Shaukat1
ABSTRACT
Objective: We compared the diagnostic performance of FIA 8000 Quantitative immunoassay point-of-care device for cardiac troponin I (TropP) with fully automated central laboratory Vitros ECiQ Immunodiagnostic Systems (TropV).
Study Design: Experimental study.
Place and Duration of Study: This study was conducted at the Emergency and Pathology Department of Quaid-e-Azam Medical College Bahawalpur over a period of 12 months from September 2015 to October 2016.
Materials and Methods: Blood specimens for cTn I measurement from patients suspected of Myocardial infarction in the ED were divided into two parts. One was analyzed on the FIA 8000. Other was analyzed on Vitros. The calculation of sensitivity, specificity, positive and negative predictive values for TropP were made. In TropP elevated samples imprecision, bias and comparative analysis were performed with respect to TropV.
Results: The specificity and false negative results for TropP were more than TropV. The kappa analysis revealed moderate agreement (κ=0.596). Of the 263 elevated TropP, 18 were negative by TropV, overall TropV results were higher. Coefficient of variations (CVs) was less than 10% in both within and between run assays. Initial comparison of results using Spearman test showed correlation coefficient (r) of 0.98. The results showed good correspondence, when Bland–Altman and Passing–Bablok regression analysis were performed.
Conclusion: The FIA 8000 is helpful in early and reliable diagnosis of myocardial infarction in emergency department.
Key Words: Diagnostic performance, Cardiac troponin I, Vitros ECiQ, FIA 8000
Citation of articles: Aslam F, Arain T, Mehmud M, Ali Z, Shaukat A. Comparison of Performance characteristics between FIA 8000 and Vitros ECiQ Analyzer for Cardiac Troponin I Estimation. Med Forum 2017;28(7):92-96.