16. Antioxidant Effects of Walnuts on Superoxide Dismutase Levels Reduced by Lead Toxicity in Mice
Roomisa Anis1, Ayesha Shafqat1, Misbah Batool2, Beenish Zafar1, Naveeda Zaigham1 and Shahzadi Ambreen3
ABSTRACT
Objective: To evaluate the antioxidant effects of walnuts on lead toxicity by estimating the superoxide dismutase levels.
Study Design: Quasi Experimental Study
Place and Duration of Study: Department of Biochemistry, ANMCH, Islamabad, Pakistan in collaboration with NIH, Islamabad from November, 2015 to April, 2016.
Materials and Methods: 60 BLAB/C mice were divided into three groups of 20 mice each. Group Ι was given normal standard diet. Group ΙΙ was given lead acetate in drinking water along with normal diet without any supplementation. Group ΙΙΙ was given lead acetate along with the diet supplemented with walnuts. Superoxide Dismutase (SOD) was estimated at the end of the study by xanthine oxidase method.
Results: Toxicity of lead caused decrease in the SOD in group ΙΙ. Supplementation of walnut along with lead showed Increase in SOD levels in group ΙΙΙ (p value ≤0.001) as compared to group ΙΙ.
Conclusion: This study concludes that antioxidants present in walnuts decrease lead induced lipid peroxidation by increasing the levels of antioxidant enzyme SOD.
Key Words: Antioxidants, Lead Toxicity, Lipid peroxidation, Superoxide Dismutase, Walnuts
Citation of article: Anis R, Shafqat A, Batool M, Zafar B, Zaigham N, Ambreen S. Antioxidant Effects of Walnuts on Superoxide Dismutase Levels Reduced by Lead Toxicity in Mice. Med Forum 2020;31(9):67-71.