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  3. 15. Protective Effect of L-Arginine on Diclofenac Mediated Renal Toxicity in Adult Albino Rats
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15. Protective Effect of L-Arginine on Diclofenac Mediated Renal Toxicity in Adult Albino Rats

Abdul Rehman Rajput1, Maria Mohiuddin2, Amtul Sughra2, Rosheena Nabeel Khan3, Azmat Ullah4 and Hemant Kumar2


Objective: The objective of this study is to observe the protective effect of L-arginine in the Diclofenac sodium in experimental animal study.

Study Design: An experimental study

Place and Duration of Study: This study was conducted at the Institute of Bio Medical Sciences (IBMS) with the cooperation of Dow Diagnostic Research and Reference Laboratory (DDRRL) in Dow University of Health Sciences (DUHS), Ojha campus from December, 2013 to January 2014.

Materials and Methods: Forty adult albino rats weighing 200 ± 20 gms were used and divided into four groups. The rats in the control group A,(n=10) each were given intra-muscular injection, one cc of physiologic saline. The second group B,(n=10), was given Diclofenac sodium 2 mg/kg body weight, intra-muscular. The rats in the third group C,(n=10) were given Diclofenac sodium 2mg/kg by intra-muscular injection and L-arginine, 1mg/kg of body weight, by feeding tube. The fourth group D,(n=10) was given L-arginine, with the ratio of 1mg/kg of body weight, by feeding tube orally. Gross and histological changes were observed in the proximal convoluted tubules (PCT) of the kidney of albino rats, after completion of the two weeks treatment.

Results: The result of present study revealed that diclofenac sodium significant decrease in body weight in adult albino rats was after 2 weeks treatment with in a single daily dose of 2mg/kg intra muscular. There were changes in absolute kidney weight, cortical thickness of kidney and diameter of proximal convoluted tubules. Treatment with L-arginine in a daily dose of 1mg/kg body weight, partially prevented the Diclofenac sodium induced damage of proximal convoluted tubules. L-arginine alone in same daily dose had no adverse effect on kidney in young  albino rats.

Conclusion: Diclofenac sodium, an NSAID induces nephrotoxicity and L-arginine when given simultaneously partially protects the kidneys from its harmful effects.

Key Words: Diclofenac sodium, L-arginine, proximal convoluted tubules

Citation of article: Rajput AR, Mohiuddin M, Sughra A, Khan RN, Azmat Ullah, Kumar H. Protective Effect of L-Arginine On Diclofenac Mediated Renal Toxicity in Adult Albino Rats Med Forum 2021;32(2):63-67.