13. Investigating Hepatoprotective Effects of Pentoxifylline in Carbon Tetrachloride Induced Liver Injury in Rat Model
Kashif Rasheed Shaikh1, Sadia Tabassum2, Shumaila Shaikh3, Shagufta Memon4, Shumail Saeed Siddiqui5 and Umair Ali Soomro6
ABSTRACT
Objective: Investigating the hepatoprotective effects of Pentoxifylline (PTX) in carbon tetrachloride (CCl4) induced liver injury in rat model.
Study Design: Experimental study
Place and Duration of Study: This study was conducted at the Animal house of Sindh Agriculture University Tando Jam and Isra University from January 2017 to August 2017.
Materials and Methods: 45 male rats were selected by purposive sampling through criteria of inclusion. Animals were housed in stainless steel cages under standard conditions. Rats were divided into Group A (control), Group B (CCl4) and Group C (CCl4 + PTX 200 mg/kg). Blood samples were centrifuged to get sera for the estimation of serum bilirubin, creatinine and liver enzymes. Liver tissue sections (3-5μ thickness) were stained with H & E and Microscopic tissue findings were noted. Statistical software SPSS version 22.0 (IBM corporation) was used for statistical analysis (P≤0.5).
Results: Serum bilirubin and creatinine in control group in experimental groups B and C were raised (P<0.05). Pentoxifylline treated group C rats revealed reduction of 65% in ALT, 58% in AST, 48% LDH and 71% ALP (P<0.05). Liver histology was also improved in PTX treated group C (P<0.05).
Conclusion: Pentoxifylline shows hepatoprotective potential against chemical induced liver injury, however mechanism of action remains to be elucidated. Key Words: Carbon tetrachloride, Rat model, Liver injury, Pentoxifylline
Citation of article: Shaikh KR, Tabassum S, Shaikh S, Memon S, Siddiqui SS, Soomro UA. Investigating Hepatoprotective Effects of Pentoxifylline in Carbon Tetrachloride Induced Liver Injury in Rat Model. Med Forum 2020;31(7):55-59.