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11. Microbiological Profile and Susceptibility Pattern of Enteric Organisms in Raw Broiler Chicken Meat from Abattoirs of Lahore, Pakistan
Nida Javed1, Saima Pervaiz2, Fatima Tuz Zahra3, Ghania Ali4 and Ayesha Muazzam4
ABSTRACT
Objective: This study aimed to estimate number of enteric pathogens in raw chicken meat including surfaces of breast and cloacal region. Moreover, antibiotics susceptibility pattern of organisms isolated from thigh meat were also observed.
Study Design: A Descriptive, cross-sectional study.
Place and Duration of Study: This study was conducted at the Department of Microbiology, University of Health Sciences (UHS) Lahore. December 2021 to April 2022.
Methods: About 95 raw broiler chicken thigh meat samples from abattoirs of different areas of Lahore were collected. Swabs from chicken breast and cloacal region were also taken to estimate the microbes present on their surface.
Results: The mean Aerobic Plate Count was 4.75+1.18 log CFU/g. Out of 95 chicken samples, thigh meat had E. coli (n= 67), Proteus spp., (n=15), Enterobacter spp., (n=6), Citrobacter spp., (n=6) and S. aureus (n=3). From breast swab, E. coli (n= 69), Proteus spp., (n=16), Enterobacter spp., (n=9), Yersinia spp., (n=1), Citrobacter spp., (n=4), S. aureus (n=9) and Klebsiella spp., (n=6) were retrieved. Cloacal swabs revealed E. coli (n= 59), Proteus spp., (n=67), Enterobacter spp., (n=5), Yersinia spp., (n=1) and Citrobacter spp., (n=2). All the isolates were resistant to ampicillin.
Conclusion: Various Enterobacteriaceae species especially E. coli are common in broiler chickens at abattoirs. The presence of multi drug resistant strains of these enteric organisms is an important finding of this study.
Key Words: Antibiotics, Cross contamination, E. coli, Enterobacteriaceae, Multidrug resistant bacteria, Poultry meat, Raw Chicken