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1. Hepatoprotective Effects of Curcuma Longa against Carbon Tetrachloride Induced Liver Injury in Rats
1. Zaheer Ahmed Memon 2. Muhammad Bachal Pandhiani 3. Aftab Ahmed Shaikh
4. Haji Khan Khoharo
1. Asstt. Prof. of Anatomy, Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad
2. Asstt. Prof. of Anatomy, Al-Tibri Medical College, Karachi 3. Asstt. Prof. of Pharmacology, Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad 4. Asstt. Prof. of Physiology/Medicine, Faculty of Medicine and Allied Medical Sciences, Isra University, Hyderabad
ABSTRACT
Objective: To investigate the protective effect of Curcuma longa (CL) against carbon tetrachloride (CCl4) induced liver injury in adult male Wistar rat model.
Study Design: Experimental/Analytical study
Place and Duration of Study: Animal House, Isra University Hyderabad from March to December 2013.
Subjects and Methods: Forty five adult male Wistar rats were divided into three groups; Group 1. controls received
0.9% isotonic saline, Group 2. received CCl4 orally (1.9mg/kg) mixed in olive oil, and Group 3. received the CCl4+CL (250mg/kg) Blood samples were collected for liver biochemical assays. The animals were sacrificed, liver tissue, after fixation in 4% formaldehyde, was embedded in paraffin. Tissue sections of 5μ thickness were subjected to haematoxylin and eosin staining and were assessed by light microscopy. The data was analyzed on SPSS 21.0 using one-way ANOVA, Fischer`s LSD and Chi-square tests. A p-value of ≤ 0.05 was taken statistically significant. Results: The liver biochemical and histological findings reveal statistically significant differences among the controls, CCl4 and CCl4+CL groups (p=0.0001). Liver enzymes and histology were deranged significantly in CCl4 group compared to controls and CCl4 +CL group (p=0.0001). The CCl4+CL group showed less elevation of liver enzymes and derangement in liver histology compared to CCl4 group (p=0.001). The histological findings of congestion, inflammatory cell infiltrate, vacuolar degeneration and necrosis are found prominent in CCl4 group. Conclusion: The Curcuma longa protects against oxidative damages caused by carbon tetrachloride induced liver injury in rat model.
Key Words: Curcuma longa, Carbon Tetrachloride, Liver injury.