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  3. 5.Effect of Pumpkin Seed Oil against High Fat Diet Induced Hyperlipidemia in Wistar Albino Rats
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5.Effect of Pumpkin Seed Oil against High Fat Diet Induced Hyperlipidemia in Wistar Albino Rats

Asim Mehmood1, Samreen Ali2, Rasheed Ahmed Soomro3, Majid Ali Hingoro5, Umair Ali Soomro6 and Muhammad Atif Ata4

ABSTRACT

Objective: To study and analyze the lipid lowering and anti – obesity effect of Pumpkin seed oil (PSO) against high fat diet induced hyperlipidemia in Wistar Albino male rats.

Study Design: Experimental study

Place and Duration of Study: This study was conducted at the Suleman Roshan Medical College, Tando Adam and Animal House of the Sindh Agriculture University, Tando Jam from April 2021 to September 2021 for a period of six months.

Materials and Methods: Sixty male Wistar albino rats were included in experiment according to the inclusion criteria. Rats were kept in animal house as per standard protocol of animal handling in Laboratory (NIH), USA guidelines. HFD was prepared as described previously. Rats were divided into four groups (A to D), each group comprised 15 rats. Pumpkin seed oil (PSO) was purchased from market. After the experiment, blood samples were collected for blood lipids biochemical analysis. Data was analyzed on SPSS 21.0 ver. (IBM, Incorporation, USA) using 1– way ANOVA, descriptive and Post – Hoc LSD at 95% confidence interval (P<0.05).

Results: Six weeks PSO therapy decreased the body weight and exerted lipid lowering potential.  Serum triglycerides (TAGs), total cholesterol (TC), serum LDL cholesterol (LDLc) and serum HDL cholesterol (HDLc) shows significant reduction compared to positive control group B (P<0.05).

Conclusion: The present study concludes the pumpkin seed oil shows lipid lowering and anti – obesity potential hence it may be used for clinical purpose of treating hyperlipidemia

Key Words: Pumpkin seed oil, Hyperlipidemia, Lipid lowering, Rats

Citation of article: Mehmood A, Ali S, Soomro RA, Hingoro MA, Soomro UA, Ata MA. Effect of Pumpkin Seed Oil against High Fat Diet Induced Hyperlipidemia in Wistar Albino Rats. Med Forum 2022;33(1):17-21.