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  3. 5. Association of rs6295 with Risk of Development of Major Depressive Disorder in Adult Diabetic Population in Context of Family History of Major Depressive Disorder
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5. Association of rs6295 with Risk of Development of Major Depressive Disorder in Adult Diabetic Population in Context of Family History of Major Depressive Disorder

Tayba Saleha Hashmi1, Amena Rahim2, Farhana Ayub3, Samarah Naeem2, Bilal Karim4 and Jaweria Maqsood5

ABSTRACT

Objective: To determine whether the presence of family history of Major Depressive Disorder (MDD) is associated with the risk of development of the disease in adult diabetic population on the basis of genetic association with single nucleotide polymorphism (SNP) rs6295.

Study Design: Case control study

Place and Duration of Study: This study was conducted at the Department of Biochemistry, Islamic International Medical College Rawalpindi in collaboration with Railway General Hospital Rawalpindi, Armed Forces Institute of Pathology (AFIP) Rawalpindi and Institute of Biomedical and Genetic Engineering (IB&GE) Islamabad. Duration of study was 1 year from September 2020 to September 2021.

Materials and Methods: A total of 400 subjects were included in the study, out of which 200 were cases and 200 were age, gender and ethnicity matched healthy controls. Out of 200 cases, 100 cases had diabetes mellitus (DM) only and 100 cases had DM with MDD. Both males and females were included in the study having ages 25 years and above. Cases of both type I and type II DM were included in the study. Blood samples were collected and DNA was extracted by Chelax method. Real-time PCR was carried out to determine respective allelic frequencies of rs6295 genotype using TaqMan SNP genotyping assays and master mix.

Results: According to our results no significant association was found between rs6295 genotype and risk of development of MDD in Pakistani diabetic population in context of family history of MDD.

Conclusion: There is no significant association of SNP rs6295 of 5-Hydroxytryptamine 1A receptor gene with MDD in Pakistani diabetics having family history of MDD.

Key Words: Diabetes mellitus, major depressive disorder, 5-hydroxytryptamine 1A receptor gene, single nucleotide polymorphism, polymerase chain reaction.

Citation of article: Hashmi TS, Rahim A, Ayub F, Naeem S, Karim B, Maqsood J. Association of rs6295 with Risk of Development of Major Depressive Disorder in Adult Diabetic Population in Context of Family History of Major Depressive Disorder. Med Forum 2022;33(8):26-29.